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A cytometry-based assay to determine optimal Janelia Fluor dye labelling of Halo and SNAP tags
When used in combination, self-labelling protein tags such as Halo, SNAP, and CLIP allow for the simultaneous visualization of proteins across a wide fluorescence spectrum. However, the combination of cell type, ligand binding and fluorescent dye chemistry introduces several variables that need to b...
Guardado en:
| Publicado en: | J Biomol Tech |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Association of Biomolecular Resource Facilities
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7424637/ |
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