Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags

Super-resolution microscopy requires that subcellular structures are labeled with bright and photostable fluorophores, especially for live-cell imaging. Organic fluorophores may help here as they can yield more photons—by orders of magnitude—than fluorescent proteins. To achieve molecular specificit...

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Publié dans:Cell Chem Biol
Auteurs principaux: Erdmann, Roman S., Baguley, Stephanie Wood, Richens, Jennifer H., Wissner, Rebecca F., Xi, Zhiqun, Allgeyer, Edward S., Zhong, Sheng, Thompson, Alexander D., Lowe, Nicholas, Butler, Richard, Bewersdorf, Joerg, Rothman, James E., St Johnston, Daniel, Schepartz, Alanna, Toomre, Derek
Format: Artigo
Langue:Inglês
Publié: Cell Press 2019
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6474801/
https://ncbi.nlm.nih.gov/pubmed/30745239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2019.01.003
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