Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags

Super-resolution microscopy requires that subcellular structures are labeled with bright and photostable fluorophores, especially for live-cell imaging. Organic fluorophores may help here as they can yield more photons—by orders of magnitude—than fluorescent proteins. To achieve molecular specificit...

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Publicado en:Cell Chem Biol
Autores principales: Erdmann, Roman S., Baguley, Stephanie Wood, Richens, Jennifer H., Wissner, Rebecca F., Xi, Zhiqun, Allgeyer, Edward S., Zhong, Sheng, Thompson, Alexander D., Lowe, Nicholas, Butler, Richard, Bewersdorf, Joerg, Rothman, James E., St Johnston, Daniel, Schepartz, Alanna, Toomre, Derek
Formato: Artigo
Lenguaje:Inglês
Publicado: Cell Press 2019
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6474801/
https://ncbi.nlm.nih.gov/pubmed/30745239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2019.01.003
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