Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags

Super-resolution microscopy requires that subcellular structures are labeled with bright and photostable fluorophores, especially for live-cell imaging. Organic fluorophores may help here as they can yield more photons—by orders of magnitude—than fluorescent proteins. To achieve molecular specificit...

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Udgivet i:Cell Chem Biol
Main Authors: Erdmann, Roman S., Baguley, Stephanie Wood, Richens, Jennifer H., Wissner, Rebecca F., Xi, Zhiqun, Allgeyer, Edward S., Zhong, Sheng, Thompson, Alexander D., Lowe, Nicholas, Butler, Richard, Bewersdorf, Joerg, Rothman, James E., St Johnston, Daniel, Schepartz, Alanna, Toomre, Derek
Format: Artigo
Sprog:Inglês
Udgivet: Cell Press 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6474801/
https://ncbi.nlm.nih.gov/pubmed/30745239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2019.01.003
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