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De novo sequence assembly requires bioinformatic checking of chimeric sequences

De novo assembly of sequence reads from next generation sequencing platforms is a common strategy for detecting presence and sequencing of viruses in biospecimens. Amplification artifacts and presence of several related viruses in the same specimen can lead to assembly of erroneous, chimeric sequenc...

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Pubblicato in:PLoS One
Autori principali: Arroyo Mühr, Laila Sara, Lagheden, Camilla, Hassan, Sadaf Sakina, Kleppe, Sara Nordqvist, Hultin, Emilie, Dillner, Joakim
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7417191/
https://ncbi.nlm.nih.gov/pubmed/32777809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0237455
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