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Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP

Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative diseases. Here, we present complementary experimental...

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書目詳細資料
發表在:Bio Protoc
Main Authors: Babinchak, W. Michael, Surewicz, Witold K.
格式: Artigo
語言:Inglês
出版: Bio-Protocol 2020
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7409826/
https://ncbi.nlm.nih.gov/pubmed/32775538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3489
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