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Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro. As a proof-of-concept application, we utilized this activity to re...
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| Veröffentlicht in: | eLife |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
eLife Sciences Publications, Ltd
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7402673/ https://ncbi.nlm.nih.gov/pubmed/32701057 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.54919 |
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