Methanol fixation and tagmentation of RNA/DNA hybrids directly enable single-cell transcriptome sequencing
ObjectiveSingle-cell transcriptome sequencing is a powerful tool for investigating cellular diversity in normal development and disease. However, prevalent methods predominantly employ 3′-end sequencing of transcripts, limiting the analysis of alternative splicing and other post-transcriptional proc...
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| Principais autores: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Frontiers Media S.A.
2025-07-01
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| Serier: | Frontiers in Genetics |
| Fag: | |
| Online adgang: | https://www.frontiersin.org/articles/10.3389/fgene.2025.1629655/full |
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