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Cell fixation and preservation for droplet-based single-cell transcriptomics

Abstract Background Recent developments in droplet-based microfluidics allow the transcriptional profiling of thousands of individual cells in a quantitative, highly parallel and cost-effective way. A critical, often limiting step is the preparation of cells in an unperturbed state, not altered by s...

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Principais autores: Jonathan Alles, Nikos Karaiskos, Samantha D. Praktiknjo, Stefanie Grosswendt, Philipp Wahle, Pierre-Louis Ruffault, Salah Ayoub, Luisa Schreyer, Anastasiya Boltengagen, Carmen Birchmeier, Robert Zinzen, Christine Kocks, Nikolaus Rajewsky
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2017-05-01
coleção:BMC Biology
Assuntos:
Droplet-based single-cell transcriptomics
Drop-seq
Gene expression profiling
Fixation
Alcohol-based fixation
Methanol
Acesso em linha:http://link.springer.com/article/10.1186/s12915-017-0383-5
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