RNA sequencing by direct tagmentation of RNA/DNA hybrids

Transcriptome profiling by RNA sequencing (RNA-seq) has been widely used to characterize cellular status, but it relies on second-strand complementary DNA (cDNA) synthesis to generate initial material for library preparation. Here we use bacterial transposase Tn5, which has been increasingly used in...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Di, Lin, Fu, Yusi, Sun, Yue, Li, Jie, Liu, Lu, Yao, Jiacheng, Wang, Guanbo, Wu, Yalei, Lao, Kaiqin, Lee, Raymond W., Zheng, Genhua, Xu, Jun, Oh, Juntaek, Wang, Dong, Xie, X. Sunney, Huang, Yanyi, Wang, Jianbin
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2020
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7022195/
https://ncbi.nlm.nih.gov/pubmed/31988135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1919800117
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