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RNA sequencing by direct tagmentation of RNA/DNA hybrids
Transcriptome profiling by RNA sequencing (RNA-seq) has been widely used to characterize cellular status, but it relies on second-strand complementary DNA (cDNA) synthesis to generate initial material for library preparation. Here we use bacterial transposase Tn5, which has been increasingly used in...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , , , , , , , , , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7022195/ https://ncbi.nlm.nih.gov/pubmed/31988135 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1919800117 |
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