Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region

The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for in vivo applications raises safety concerns due to the presence of SV40 T antigen-encoding sequences. We used CRISPR-Cas9 genome editing to remove the SV40 T antigen-encoding sequences from HEK293T cells by transfe...

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Detalhes bibliográficos
Publicado no:Mol Ther Methods Clin Dev
Main Authors: Bae, Dahae Hailey, Marino, Michael, Iaffaldano, Brian, Fenstermaker, Sydney, Afione, Sandra, Argaw, Takele, McCright, Jacob, Kwilas, Anna, Chiorini, John A., Timmons, Andrew E., Reiser, Jakob
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Gene & Cell Therapy 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7397404/
https://ncbi.nlm.nih.gov/pubmed/32775497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2020.07.006
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