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CRISPR Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly
CRISPR Cas9 genome editing allows researchers to modify genesin a multitude of ways including to obtain deletions, epitope-tagged loci, and knock-in mutations. Within six years of its initial application, CRISPR Cas9 genome editing has become widely employed, but disadvantages to this method, such a...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7391466/ https://ncbi.nlm.nih.gov/pubmed/32006411 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0290-4_20 |
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