Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies

Circular yeast artificial chromosomes (YACs) provide significant advantages for cloning and manipulating large segments of genomic DNA in Saccharomyces cerevisiae. However, it has been difficult to exploit these advantages, because circular YACs are difficult to isolate and purify. Here we describe...

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Detalles Bibliográficos
Publicado en:Nat Protoc
Main Authors: Noskov, Vladimir N, Chuang, Ray-Yuan, Gibson, Daniel G, Leem, Sun-Hee, Larionov, Vladimir, Kouprina, Natalay
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7380547/
https://ncbi.nlm.nih.gov/pubmed/21212778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.174
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