Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies

Circular yeast artificial chromosomes (YACs) provide significant advantages for cloning and manipulating large segments of genomic DNA in Saccharomyces cerevisiae. However, it has been difficult to exploit these advantages, because circular YACs are difficult to isolate and purify. Here we describe...

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Detaylı Bibliyografya
Yayımlandı:Nat Protoc
Asıl Yazarlar: Noskov, Vladimir N, Chuang, Ray-Yuan, Gibson, Daniel G, Leem, Sun-Hee, Larionov, Vladimir, Kouprina, Natalay
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2010
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7380547/
https://ncbi.nlm.nih.gov/pubmed/21212778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.174
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