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Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies
Circular yeast artificial chromosomes (YACs) provide significant advantages for cloning and manipulating large segments of genomic DNA in Saccharomyces cerevisiae. However, it has been difficult to exploit these advantages, because circular YACs are difficult to isolate and purify. Here we describe...
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| Publié dans: | Nat Protoc |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7380547/ https://ncbi.nlm.nih.gov/pubmed/21212778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.174 |
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