Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies

Circular yeast artificial chromosomes (YACs) provide significant advantages for cloning and manipulating large segments of genomic DNA in Saccharomyces cerevisiae. However, it has been difficult to exploit these advantages, because circular YACs are difficult to isolate and purify. Here we describe...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nat Protoc
Egile Nagusiak: Noskov, Vladimir N, Chuang, Ray-Yuan, Gibson, Daniel G, Leem, Sun-Hee, Larionov, Vladimir, Kouprina, Natalay
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2010
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7380547/
https://ncbi.nlm.nih.gov/pubmed/21212778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.174
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