Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilitating corr...

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發表在:AMB Express
Main Authors: Ghamghami, Elham, Abri Aghdam, Marjan, Tohidkia, Mohammad Reza, Ahmadikhah, Asadollah, Khanmohammadi, Morteza, Mehdipour, Tayebeh, Mokhtarzadeh, Ahad, Baradaran, Behzad
格式: Artigo
語言:Inglês
出版: Springer Berlin Heidelberg 2020
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Original Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7371774/
https://ncbi.nlm.nih.gov/pubmed/32691183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-020-01063-x
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