Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilitating corr...

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Dettagli Bibliografici
Pubblicato in:AMB Express
Autori principali: Ghamghami, Elham, Abri Aghdam, Marjan, Tohidkia, Mohammad Reza, Ahmadikhah, Asadollah, Khanmohammadi, Morteza, Mehdipour, Tayebeh, Mokhtarzadeh, Ahad, Baradaran, Behzad
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Berlin Heidelberg 2020
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Original Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7371774/
https://ncbi.nlm.nih.gov/pubmed/32691183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-020-01063-x
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