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CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries

The systematic perturbation of genomes using CRISPR/Cas9 deciphers gene function at an unprecedented rate, depth and ease. Commercially available sgRNA libraries typically contain tens of thousands of pre-defined constructs, resulting in a complexity challenging to handle. In contrast, custom sgRNA...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Becker, Martin, Noll-Puchta, Heidi, Amend, Diana, Nolte, Florian, Fuchs, Christiane, Jeremias, Irmela, Braun, Christian J
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7367185/
https://ncbi.nlm.nih.gov/pubmed/32479629
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa459
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