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CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries
The systematic perturbation of genomes using CRISPR/Cas9 deciphers gene function at an unprecedented rate, depth and ease. Commercially available sgRNA libraries typically contain tens of thousands of pre-defined constructs, resulting in a complexity challenging to handle. In contrast, custom sgRNA...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7367185/ https://ncbi.nlm.nih.gov/pubmed/32479629 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa459 |
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