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Incorporation of unique molecular identifiers in TruSeq adapters improves the accuracy of quantitative sequencing

Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference- based ali...

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Detalles Bibliográficos
Publicado en:Biotechniques
Main Authors: Hong, Jungeui, Gresham, David
Formato: Artigo
Idioma:Inglês
Publicado: 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7359820/
https://ncbi.nlm.nih.gov/pubmed/29185922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114608
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