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Incorporation of unique molecular identifiers in TruSeq adapters improves the accuracy of quantitative sequencing

Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference- based ali...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Biotechniques
Egile Nagusiak: Hong, Jungeui, Gresham, David
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2017
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7359820/
https://ncbi.nlm.nih.gov/pubmed/29185922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114608
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