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Incorporation of unique molecular identifiers in TruSeq adapters improves the accuracy of quantitative sequencing

Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference- based ali...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Hong, Jungeui, Gresham, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7359820/
https://ncbi.nlm.nih.gov/pubmed/29185922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114608
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