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Incorporation of unique molecular identifiers in TruSeq adapters improves the accuracy of quantitative sequencing
Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference- based ali...
Shranjeno v:
| izdano v: | Biotechniques |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2017
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7359820/ https://ncbi.nlm.nih.gov/pubmed/29185922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114608 |
| Oznake: |
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