Quantitative Bias in Illumina TruSeq and a Novel Post Amplification Barcoding Strategy for Multiplexed DNA and Small RNA Deep Sequencing

Here we demonstrate a method for unbiased multiplexed deep sequencing of RNA and DNA libraries using a novel, efficient and adaptable barcoding strategy called Post Amplification Ligation-Mediated (PALM). PALM barcoding is performed as the very last step of library preparation, eliminating a potenti...

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Main Authors: Van Nieuwerburgh, Filip, Soetaert, Sandra, Podshivalova, Katie, Ay-Lin Wang, Eileen, Schaffer, Lana, Deforce, Dieter, Salomon, Daniel R., Head, Steven R., Ordoukhanian, Phillip
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2011
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3203936/
https://ncbi.nlm.nih.gov/pubmed/22046424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0026969
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