Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling

Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect...

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Bibliografiske detaljer
Udgivet i:Int J Mol Sci
Main Authors: De Luca, Giuseppa, Cardinali, Barbara, Del Mastro, Lucia, Lastraioli, Sonia, Carli, Franca, Ferrarini, Manlio, Calin, George A., Garuti, Anna, Mazzitelli, Carlotta, Zupo, Simona, Dono, Mariella
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7352435/
https://ncbi.nlm.nih.gov/pubmed/32575430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21124364
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