Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling

Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: De Luca, Giuseppa, Cardinali, Barbara, Del Mastro, Lucia, Lastraioli, Sonia, Carli, Franca, Ferrarini, Manlio, Calin, George A., Garuti, Anna, Mazzitelli, Carlotta, Zupo, Simona, Dono, Mariella
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7352435/
https://ncbi.nlm.nih.gov/pubmed/32575430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21124364
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