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Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM

Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvatur...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Cells
Hauptverfasser: Begemann, Isabell, Keller, Ulrike, Nüsse, Harald, Klingauf, Jürgen, Galic, Milos
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7349049/
https://ncbi.nlm.nih.gov/pubmed/32466457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9061329
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