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Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM

Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvatur...

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Publicat a:Cells
Autors principals: Begemann, Isabell, Keller, Ulrike, Nüsse, Harald, Klingauf, Jürgen, Galic, Milos
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7349049/
https://ncbi.nlm.nih.gov/pubmed/32466457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9061329
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