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Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM

Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvatur...

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Detalhes bibliográficos
Publicado no:Cells
Main Authors: Begemann, Isabell, Keller, Ulrike, Nüsse, Harald, Klingauf, Jürgen, Galic, Milos
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7349049/
https://ncbi.nlm.nih.gov/pubmed/32466457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9061329
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