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SDS PAGE to immunoblot in one hour

An ultra-rapid method for electrophoresing proteins on SDS PAGE, transfer of proteins to nitrocellulose membranes and immunoblotting is described here. Electrophoresis of the autoantigens La and Ro60, as well as molecular weight standards on a 4–20% gradient gel was performed in about 10 minutes usi...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Kurien, Biji T., Danda, Debashish, Bachmann, Michael, Scofield, R. Hal
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7346231/
https://ncbi.nlm.nih.gov/pubmed/26044026
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2694-7_45
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