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Analysis of antibody clonotype by affinity immunoblotting

A sensitive and specific method to analyze specific antibody clonotype changes in a lupus patient who developed autoantibodies to the Ro 60 autoantigen under observation is described. Patient sera collected over several years were separated by flatbed isoelectric focusing (IEF) and analyzed by affin...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Kurien, Biji T., Scofield, R. Hal
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7304523/
https://ncbi.nlm.nih.gov/pubmed/26043996
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2694-7_15
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