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Analysis of antibody clonotype by affinity immunoblotting

A sensitive and specific method to analyze specific antibody clonotype changes in a lupus patient who developed autoantibodies to the Ro 60 autoantigen under observation is described. Patient sera collected over several years were separated by flatbed isoelectric focusing (IEF) and analyzed by affin...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Kurien, Biji T., Scofield, R. Hal
Format: Artigo
Sprog:Inglês
Udgivet: 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7304523/
https://ncbi.nlm.nih.gov/pubmed/26043996
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2694-7_15
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