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SDS PAGE to immunoblot in one hour
An ultra-rapid method for electrophoresing proteins on SDS PAGE, transfer of proteins to nitrocellulose membranes and immunoblotting is described here. Electrophoresis of the autoantigens La and Ro60, as well as molecular weight standards on a 4–20% gradient gel was performed in about 10 minutes usi...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7346231/ https://ncbi.nlm.nih.gov/pubmed/26044026 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2694-7_45 |
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