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Optimization of western blotting for the detection of proteins of different molecular weight
Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended...
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| Publicado no: | Biotechniques |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Future Science Ltd
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7333534/ https://ncbi.nlm.nih.gov/pubmed/32283940 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2019-0124 |
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