Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Mucins, the heavily-glycosylated proteins lining mucosal surfaces, have evolved as a key component of innate defense by protecting the epithelium against invading pathogens. The main role of these macromolecules is to facilitate particle trapping and clearance while promoting lubrication of the muco...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Ramsey, Kathryn A., Rushton, Zachary L., Ehre, Camille
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2016
Subjects:
Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4927784/
https://ncbi.nlm.nih.gov/pubmed/27341489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54153
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