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Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Mucins, the heavily-glycosylated proteins lining mucosal surfaces, have evolved as a key component of innate defense by protecting the epithelium against invading pathogens. The main role of these macromolecules is to facilitate particle trapping and clearance while promoting lubrication of the muco...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Ramsey, Kathryn A., Rushton, Zachary L., Ehre, Camille
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4927784/
https://ncbi.nlm.nih.gov/pubmed/27341489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54153
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