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Effects of Reusing Gel Electrophoresis and Electrotransfer Buffers on Western Blotting

SDS-PAGE and Western blotting are 2 of the most commonly used biochemical methods for protein analysis. Proteins are electrophoretically separated based on their MWs by SDS-PAGE and then electrotransferred to a solid membrane surface for subsequent protein-specific analysis by immunoblotting, a proc...

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Detalles Bibliográficos
Publicado en:J Biomol Tech
Autores principales: Heda, Ghanshyam D., Omotola, Oluwabukola B., Heda, Rajiv P., Avery, Jamie
Formato: Artigo
Lenguaje:Inglês
Publicado: Association of Biomolecular Resource Facilities 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4972471/
https://ncbi.nlm.nih.gov/pubmed/27582639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.16-2703-004
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