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Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins
The autocatalytic process of protein splicing is facilitated by an intein, which interrupts flanking polypeptides called exteins. The mechanism of protein splicing has been studied by overexpression in E. coli of intein fusion proteins with nonnative exteins. Inteins can be used to generate reactive...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7325523/ https://ncbi.nlm.nih.gov/pubmed/32144663 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0434-2_4 |
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