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pK(a) Coupling at the Intein Active Site: Implications for the Coordination Mechanism of Protein Splicing with a Conserved Aspartate

[Image: see text] Protein splicing is a robust multistep posttranslational process catalyzed by inteins. In the Mtu RecA intein, a conserved block-F aspartate (D422) coordinates different steps in protein splicing, but the precise mechanism is unclear. Solution NMR shows that D422 has a strikingly h...

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Bibliografske podrobnosti
Main Authors: Du, Zhenming, Zheng, Yuchuan, Patterson, Melissa, Liu, Yangzhong, Wang, Chunyu
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3126871/
https://ncbi.nlm.nih.gov/pubmed/21604815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja203209f
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