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Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins
The autocatalytic process of protein splicing is facilitated by an intein, which interrupts flanking polypeptides called exteins. The mechanism of protein splicing has been studied by overexpression in E. coli of intein fusion proteins with nonnative exteins. Inteins can be used to generate reactive...
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7325523/ https://ncbi.nlm.nih.gov/pubmed/32144663 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0434-2_4 |
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