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A Generic Procedure for the Isolation of pH- and Magnesium-Responsive Chicken scFvs for Downstream Purification of Human Antibodies

Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs). Protein A chromatography constitutes the gold standard for Mab purification. However, the required acidic conditi...

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Bibliografski detalji
Izdano u:Front Bioeng Biotechnol
Glavni autori: Hinz, Steffen C., Elter, Adrian, Rammo, Oliver, Schwämmle, Achim, Ali, Ataurehman, Zielonka, Stefan, Herget, Thomas, Kolmar, Harald
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7324474/
https://ncbi.nlm.nih.gov/pubmed/32656201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.00688
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