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Directed evolution of human scFvs in DT40 cells

Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and for refining existing affinities and specificities. Here, we report an adaptation of the chicken DT40 system wherein its capacity for somatic hypermutation is harnessed to evolve human an...

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Detalhes bibliográficos
Publicado no:Protein Eng Des Sel
Main Authors: Lim, Alfred W.Y., Williams, Gareth T., Rada, Cristina, Sale, Julian E.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4840456/
https://ncbi.nlm.nih.gov/pubmed/26519451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzv058
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