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Designer Receptors for Nucleotide‐Resolution Analysis of Genomic 5‐Methylcytosine by Cellular Imaging

We report programmable receptors for the imaging‐based analysis of 5‐methylcytosine (5mC) in user‐defined DNA sequences of single cells. Using fluorescent transcription‐activator‐like effectors (TALEs) that can recognize sequences of canonical and epigenetic nucleobases through selective repeats, we...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Muñoz‐López, Álvaro, Buchmuller, Benjamin, Wolffgramm, Jan, Jung, Anne, Hussong, Michelle, Kanne, Julian, Schweiger, Michal R., Summerer, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7318601/
https://ncbi.nlm.nih.gov/pubmed/32167219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202001935
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