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Intact and middle‐down CIEF of commercial therapeutic monoclonal antibody products under non‐denaturing conditions
A two‐step CIEF with chemical mobilization was developed for charge profiling of the therapeutic mAb rituximab under non‐denaturing separation conditions. CIEF of the intact mAb was combined with a middle‐down approach analyzing Fc/2 and F(ab´)(2) fragments after digest with a commercial cysteine pr...
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| Publicado no: | Electrophoresis |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7317833/ https://ncbi.nlm.nih.gov/pubmed/32250465 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.202000013 |
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