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Tuning Enzyme Activity for Nonaqueous Solvents: Engineering an Enantioselective “Michaelase” for Catalysis in High Concentrations of Ethanol

Enzymes have evolved to function under aqueous conditions and may not exhibit features essential for biocatalytic application, such as the ability to function in high concentrations of an organic solvent. Consequently, protein engineering is often required to tune an enzyme for catalysis in non‐aque...

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書目詳細資料
發表在:Chembiochem
Main Authors: Guo, Chao, Biewenga, Lieuwe, Lubberink, Max, van Merkerk, Ronald, Poelarends, Gerrit J.
格式: Artigo
語言:Inglês
出版: John Wiley and Sons Inc. 2020
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7317446/
https://ncbi.nlm.nih.gov/pubmed/31886617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900721
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