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Computational and structure-guided design of phosphoinositide substrate specificity into the tyrosine specific LMW-PTP enzyme

We have used a combination of computational and structure-based redesign of the low molecular weight protein tyrosine phosphatase, LMW-PTP, to create new activity towards phosphoinositide substrates for which the wild-type enzyme had little or no activity. The redesigned enzymes retain catalytic act...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Egbe, Eyong, Levy, Colin W., Tabernero, Lydia
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7316235/
https://ncbi.nlm.nih.gov/pubmed/32584877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0235133
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