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An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

BACKGROUND: A neutral, heat-sensitive serine protease (NHSSP) originating from the feather-degrading fungus Onygena corvina (O. corvina) was described and defined as an alkaline serine protease of the subtilisin type S8 family, exhibiting an enzymatic activity at neutral pH. Generally, broad specifi...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Skowron, Piotr M., Krefft, Daria, Brodzik, Robert, Kasperkiewicz, Paulina, Drag, Marcin, Koller, Klaus-Peter
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7313183/
https://ncbi.nlm.nih.gov/pubmed/32580707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01392-3
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