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Fractionation of Enriched Phosphopeptides Using pH/Acetonitrile-Gradient-Reversed-Phase Microcolumn Separation in Combination with LC–MS/MS Analysis

Mass spectrometry (MS) is a powerful and sensitive method often used for the identification of phosphoproteins. However, in phosphoproteomics, there is an identified need to compensate for the low abundance, insufficient ionization, and suppression effects of non-phosphorylated peptides. These may h...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Ondrej, Martin, Rehulka, Pavel, Rehulkova, Helena, Kupcik, Rudolf, Tichy, Ales
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7312998/
https://ncbi.nlm.nih.gov/pubmed/32492839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21113971
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