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Fast and Efficient IMAC Protocol for Phosphopeptide enrichment for phosphoproteomic Studies via LC-MS/MS

Recent developments in first dimension High-Performance Liquid Chromatography (HPLC) separation of complex peptide mixtures, followed by a subsequent immobilized metal ion affinity chromatography (IMAC) for phosphopeptide enrichment have shown great promise in both selectivity and quantification of...

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Detalhes bibliográficos
Main Authors: McKennan, C., Spruce, L., Seeholzer, S., Ding, H.
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4162229/
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