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Fast and Efficient IMAC Protocol for Phosphopeptide enrichment for phosphoproteomic Studies via LC-MS/MS
Recent developments in first dimension High-Performance Liquid Chromatography (HPLC) separation of complex peptide mixtures, followed by a subsequent immobilized metal ion affinity chromatography (IMAC) for phosphopeptide enrichment have shown great promise in both selectivity and quantification of...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Association of Biomolecular Resource Facilities
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4162229/ |
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