A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs

RNAs that are 5′-truncated versions of a longer RNA but share the same 3′ terminus can be generated by alternative promoters in transcription of cellular mRNAs or by replicating RNA viruses. These truncated RNAs cannot be distinguished from the longer RNA by a simple two-primer RT-PCR because primer...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Kanodia, Pulkit, Prasanth, K. Reddisiva, Roa-Linares, Vicky C., Bradrick, Shelton S., Garcia-Blanco, Mariano A., Miller, W. Allen
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2020
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7297113/
https://ncbi.nlm.nih.gov/pubmed/32238481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.074963.120
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