A rapid and simple quantitative method for specific detection of smaller coterminal RNA by PCR (DeSCo-PCR): application to the detection of viral subgenomic RNAs

RNAs that are 5′-truncated versions of a longer RNA but share the same 3′ terminus can be generated by alternative promoters in transcription of cellular mRNAs or by replicating RNA viruses. These truncated RNAs cannot be distinguished from the longer RNA by a simple two-primer RT-PCR because primer...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:RNA
Egile Nagusiak: Kanodia, Pulkit, Prasanth, K. Reddisiva, Roa-Linares, Vicky C., Bradrick, Shelton S., Garcia-Blanco, Mariano A., Miller, W. Allen
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Cold Spring Harbor Laboratory Press 2020
Gaiak:
Method
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7297113/
https://ncbi.nlm.nih.gov/pubmed/32238481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.074963.120
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