Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

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Bibliografske podrobnosti
izdano v:Viruses
Main Authors: Crawford, Katharine H. D., Eguia, Rachel, Dingens, Adam S., Loes, Andrea N., Malone, Keara D., Wolf, Caitlin R., Chu, Helen Y., Tortorici, M. Alejandra, Veesler, David, Murphy, Michael, Pettie, Deleah, King, Neil P., Balazs, Alejandro B., Bloom, Jesse D.
Format: Artigo
Jezik:Inglês
Izdano: MDPI 2020
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7291041/
https://ncbi.nlm.nih.gov/pubmed/32384820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v12050513
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