Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

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Vydáno v:Viruses
Hlavní autoři: Crawford, Katharine H. D., Eguia, Rachel, Dingens, Adam S., Loes, Andrea N., Malone, Keara D., Wolf, Caitlin R., Chu, Helen Y., Tortorici, M. Alejandra, Veesler, David, Murphy, Michael, Pettie, Deleah, King, Neil P., Balazs, Alejandro B., Bloom, Jesse D.
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2020
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7291041/
https://ncbi.nlm.nih.gov/pubmed/32384820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v12050513
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