An optimized dissociation protocol for FACS-based isolation of rare cell types from Caenorhabditis elegans L1 larvae

Single-cell isolation and transcriptomic analysis of a specific cell type or tissue offers the possibility of studying cell function and heterogeneity in time-dependent processes with remarkable resolution. The reduced tissue complexity and highly stereotyped development of Caenorhabditis elegans, c...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Fernandes Póvoa, Euclides E., Ebbing, Annabel L.P., Betist, Marco C., van der Veen, Christa, Korswagen, Hendrik C.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Biochemistry, Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7265044/
https://ncbi.nlm.nih.gov/pubmed/32509539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.100922
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