Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography

RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode Caenorhabditis elegans to generate anteroposterior gene expression maps at near-cellular resolution. Here, we provide a detailed overview of the...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Autores principales: Schild, Erik S., Mars, Jonas, Ebbing, Annabel, Vivié, Judith, Betist, Marco, Korswagen, Hendrik C.
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8044689/
https://ncbi.nlm.nih.gov/pubmed/33870220
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100411
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