Integrating gene delivery and gene-editing technologies by adenoviral vector transfer of optimized CRISPR-Cas9 components

Enhancing the intracellular delivery and performance of RNA-guided CRISPR-Cas9 nucleases (RGNs) remains in demand. Here, we show that nuclear translocation of commonly used Streptococcus pyogenes Cas9 (SpCas9) proteins is suboptimal. Hence, we generated eCas9.4NLS by endowing the high-specificity eS...

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Vydáno v:Gene Ther
Hlavní autoři: Maggio, Ignazio, Zittersteijn, Hidde A., Wang, Qian, Liu, Jin, Janssen, Josephine M., Ojeda, Ivan Toral, van der Maarel, Silvère M., Lankester, Arjan C., Hoeben, Rob C., Gonçalves, Manuel A. F. V.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2020
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7253353/
https://ncbi.nlm.nih.gov/pubmed/31900423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41434-019-0119-y
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