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Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Manipulating genes in hematopoietic stem cells using conventional transgenesis approaches can be time-consuming, expensive, and challenging. Benefiting from advances in genome editing technology and lentivirus-mediated transgene delivery systems, an efficient and economical method is described here...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Sano, Soichi, Wang, Ying, Evans, Megan A., Yura, Yoshimitsu, Sano, Miho, Ogawa, Hayato, Horitani, Keita, Doviak, Heather, Walsh, Kenneth
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7249700/
https://ncbi.nlm.nih.gov/pubmed/31633690
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59977
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